What is rlec

To provide insight into the derivation of RLEC, we immunoisolated serosal liver mesothelial cells LMC and bile duct epithelial cells and attempted to propagate each epithelial cell population using culture conditions routinely employed to establish RLEC lines.

Livers were collagenase dissociated, the mesothelial capsule was "peeled" and digested with pronase to destroy contaminating hepatocytes, and rare biliary ductal epithelial cells were immunodepleted using OC.

LMC were subsequently isolated by selective binding to magnetic beads adsorbed with BD. Proliferating BD. In contrast, attempts to establish cell lines from purified OC.

Results from reverse transcriptase polymerase chain reaction analysis confirmed that LMC expressed Wilms' tumor transcripts, a lineage marker for mesodermally-derived cells. Full text is available as a scanned copy of the original print version. Get a printable copy PDF file of the complete article 3. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. National Center for Biotechnology Information , U.

Journal List Am J Pathol v. Am J Pathol. Faris , A. McBride , L. Yang , S. Affigne , C. Walker , and C. Author information Copyright and License information Disclaimer. Copyright notice. This article has been cited by other articles in PMC. Abstract Although rat liver epithelial cell RLEC lines have been developed by a number of laboratories, the identity of the clonogenic nonparenchymal progenitors is unknown.

Images in this article Figure 1 on p. Figure 2 on p. Figure 3 on p. Figure 4 on p. Figure 5 on p. Figure 6 on p.

Grisham JW. Cell types in long-term propagable cultures of rat liver. Ann N Y Acad Sci. Cell types in rat liver cultures: their identification and isolation. Mol Cell Biochem. Cultures from adult rat liver cells. Establishment of monolayer cell-cultures from normal liver. J Cell Physiol. Demonstration of organ-specific cell surface antigens on cultured cells from normal liver. You can see an example in the screenshot below. In the Node Configuration page you can see additional node related information and metrics, such as:.

Last but not least, you can view similar graphs and metrics for the cluster as a whole. The cluster is a logical grouping of all of the nodes that make it up. As you can see, this page displays similar information as in the Node Configuration page, but here it is summed up for the cluster as a whole.

I hope that through these screenshots and explanations you got a taste of the wealth and breadth of information that can be easily obtained from the RLEC UI. Such information enables you to understand exactly what is going on in the cluster, the nodes, the databases, and even each and every shard. BTW, you can also view a webinar with my colleague and Redis guru, Itamar Haber, explaining the same concept.

By continuing to use this site, you consent to our updated privacy agreement. You can change your cookie settings at any time but parts of our site will not function correctly without them. Redis Enterprise Cloud provides complete automation of day-to-day database operations. Start now with 30MB of free storage. Redis and the cube logo are registered trademarks of Redis Ltd. Itai Raz September 1, Excel will compute all the correction coefficients and provision has been made in the code to hold calibration array in memory.

Non-destructive probing active SMT components. Electrically bipolar transistor checks out as two diodes but because such combination will have current gain of one, identifier is able to distinguish between diodes and transistors. One convenient feature of PCAN is decoding pop-up window when I hover cursor over raw CAN message - this helps debugging the software: if you see wrong value, you can pinpoint bad message.

Would be impossible by just looking at raw hex numbers. Input voltage was ramped up from a power supply in discrete 0. When RLEC circuit is powered up, it continuously checks integrity of cells connection by applying short shunting pulses to each cell in round robin fashion and measuring voltage changes there should not be any difference.

This can be observed on test LEDs that blink in sequence regardless if a cell is shunted or not. Shunting is done by increasing duty cycle of these pulses, it is not continuous "on". Below is oscillogram of the voltage across shunting resistors. As I mentioned before, after receiving proper command RLECs are capable of balancing 12 cells connected to them absolutely autonomously, without any further communication with master or any CAN traffic at all.

Left side - shunt off. Right side - shunt on. This video captures RLEC shunting cell 1. This clip shows autonomous balancing executed by RLEC's firmware. I've done quite exhaustive testing of RLEC, and by now figured everything I need to be able to take advantage of it, including re-assigning its ID - few bytes command to do it is far easier than swapping out individual RLEC boards in the pack.

Next - how to turn on relay. Home of the project. Another reason is utter technical ignorance of some people out there who tried to use hardware I supplied whether my own designs or OEM , ruined it, and promptly published review blaming everyone for their failures but themselves.

Thanks to those, unfortunately I have to limit distribution of detailed technical info to only qualified OEM engineers who are welcomed to contact me if you want to engage in technical discussion or have a project on hand you need assistnace with.